Separation examples which use the New JO Chromatographic Separation System.
Separation of vitamin E (α, β, γ, δ-tocopherol) from purified scum

These are the results of a vitamin E separation test done using a pilot plant.

Test Equipment: Inside Diameter of Column 108 [mm] * Height 1200 [mm] * 12 columns

Adsorbent Capacity: 133 [l]

Separation Conditions

Adsorbent
Silica Gel (Average Pore 70Å and 30～60 Mesh)
Desorbents
Toluene/IPA = 99.5/0.5
Feed
Purified Scum
Feed Composition [%]
Others
α-tocopherol
β, γ-tocopherol
δ-tocopherol
sterol
12.0
7.0
24.0
7.0
50.0
Feed Flow Rate
0.030 [L/L-R/H]

Separation Result

[wt%]
Others
α-tocopherol
β, γ-tocopherol
δ-tocopherol
sterol
Fraction A
Purity Recovery Rate
97.6
97.4
1.1
1.8
0.0
0.0
0.0
0.0
3.0
0.3
Fraction B
Purity Recovery Rate
0.4
1.2
17.9
98.2
62.5
100.0
17.9
98.5
0.3
1.0
Fraction C
Purity Recovery Rate
3
1.4
0
0.0
0
0.0
2
1.5
99.5
98.7

As shown in the table, vitamin E (α, β, γ, δ-tocopherol) was fractionized (Fraction B) at a high purity and high recovery rate.

In this example, tocopherol was separated using silica gel as adsorbent and toluene as desorbent; however, tocopherol can also be separated using ODS silica as adsorbent and methanol as desorbent. In the case presented here, the flow was such that the higher polarity substances were withdrawn as component A, and substances of lower polarity were withdrawn as component C.

Japanese Patent Laid-Open No. 59647 (1996): "Tokohuerouru no seizou houhou (The Production Method of Tocopherol)"


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